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Standardization of in vitro male bud culture in banana musa (AA) 'kadali'
The experiment entitled “Standardization of in vitro male bud culture in banana Musa (AA) ‘Kadali’” was taken up with the objective of standardizing a protocol for mass multiplication of banana Musa (AA) ‘Kadali’ through in vitro male bud culture. The work was carried out in the Plant Tissue Culture...
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| Format: | Ph.D Thesis |
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Vellanikkara
Department of Fruit Science, College of Horticulture
2018
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| Summary: | The experiment entitled “Standardization of in vitro male bud culture in
banana Musa (AA) ‘Kadali’” was taken up with the objective of standardizing a
protocol for mass multiplication of banana Musa (AA) ‘Kadali’ through in vitro
male bud culture. The work was carried out in the Plant Tissue Culture Laboratory
at Banana Research Station, Kannara during 2016-2018. Active meristems of male
flowers were used as the explant. The study was undertaken in three parts (1)
standardization of establishment media (2) standardization of multiple shoot
induction media (3) in vitro rooting and acclimatization.
The results indicated that full MS medium containing a combination of
NAA 1.0 mgL-1 and BA 4.0 mgL-1 was suitable for better and faster explant
establishment. In multiple shoot induction media, multiple shoots were found to
develop in all the cultures with full MS medium containing BA 4.0 mgL-1 and BA
6.0 mgL-1, and the highest number of shoots were recorded in full MS medium
supplemented with NAA 1.0 mgL-1 + BA 4.0 mgL-1. Considering the length of
micro shoots and number of leaves, full MS + NAA 1.0 mgL-1 + BA 6.0 mgL-1 and
full MS + NAA 1.0 mgL-1 + BA 4.0 mgL-1 were found to be the best.
The best media identified from multiple shoot induction cultures (full MS+
NAA 1.0 mgL-1 + BA 4.0 mgL-1 and full MS + NAA 1.0 mgL-1 + BA 6.0 mgL-1)
were modified by adding different levels of thidiazuron (TDZ) and coconut water
(CW). Highest number of shoots were observed in full MS + NAA 1.0 mgL-1 + BA
4.0 mgL-1 + CW 15 % + TDZ 0.4 mgL-1and full MS + NAA 1.0 mgL-1 + BA 6.0
mgL-1+ CW 15 % + TDZ 0.4 mgL-1. With regard to number of leaves, full MS +
NAA 1.0 mgL-1 + BA 6.0 mgL-1 + CW 15 % + TDZ 0.4 mgL-1and full MS + NAA
1.0 mgL-1 + BA 4.0 mgL-1+ CW 15 % + TDZ 0.2 mgL-1 recorded the highest value.
Length of micro shoots did not show any significant difference.
In in vitro rooting studies, early rooting and significantly higher number of
roots were recorded in full MS medium with three per cent sucrose and IBA 1.0
mgL-1. Root length was highest in the combination of half MS with 1.5 per cent
sucrose and IBA 3.0 mgL-1.
Well rooted plants were hardened after potting in pro trays containing 1:1
(v/v) sterilized coco peat and vermiculite. After one month of planting out, 90 per
cent of the plants were found to survive.
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| Physical Description: | 54p |